I just upgraded from the ISO just fine yesterday from 22.214.171.124 to 126.96.36.199.
As far as the firmware, I know the old firmware doesn't work with 188.8.131.52, I am not sure about the other way around. I would say updating the AP firmware after the vWLan software is the safest bet.
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gregc, Thanks for the reply, this does answer my questions.
I just want to make a clarification here.
The OVA is used for deploying a new vWLAN.
The IMG file is used for upgrading an existing vWLAN deployment.
If you are upgrading from vWLAN 2.4 to vWLAN 2.5, then you should use the IMG file. Browse to Administration > Platform Upgrade, check the box to maintain your configuration, load the IMG file, and run the task. Afterwards you have a pending platform task to switch partitions. When you are done, you will have vWLAN 2.4 on the alternative partition. That way if you for some reason need to revert back, you can do so easily. You can find more detailed directions in the release notes for each version.